Studying the suppressing effect of mesenchymal stem cells derived from amniotic membrane on colorectal cancer

Niloofar Mesbah, Nooshin Barikrow, Masoumeh Heshmati


Mesenchymal stem cell therapy is considered as a proper tool for biological activities and treatment of diseases and cancer. Here, the suppressing effect of mesenchymal stem cells derived from the amniotic membrane (AM-MSCs) on colorectal cancer (HT29 cell line) was studied. MSCs were isolated from the human amniotic membrane and identity tests were performed. AM-MSC and HT29 cells were co-cultured and MTT assay was used to determine proper inhibitory concentration. The apoptotic effect of AM-MSC on HT29 cells was studied by Acridine orange staining. Expression of CDK2 protein and the antioxidant effect of AM-MSCs on HT29 was performed by immunocyto chemistry test. The effect of MSC on the HT29 cell line migration was determined by Scratch test. The result showed that AM-MSC had proliferation-inhibitory effects and caused apoptosis of the HT29 cell line. Real Time-PCR assay showed the increase of gene expression of p53, Caspase3 in apoptosis, and p21 in tumor cell line treated with AM-MSC. AM-MSC arrested the cell cycle of tumor cells by reduction of CDK2 protein. The migration of HT29 and ROS expression levels was decreased. Altogether, AM-MSC may play a role in suppressing colorectal cancer HT29 cell line by inhibiting angiogenesis, cell cycle, and induction of apoptosis and has antioxidant properties.


Amniotic membrane; Colorectal cancer; Co-culture; Mesenchymal stem cell


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