Histopathological changes of spermatogenesis induced by intra-peritoneal injection of CuO nanoparticles

Ghazaleh Larijani, Mahsa Motamed, Arezoo Maleki-Hajiagha, Kazem Parivar, Nasim Hayati Roodbari, Sima Vaez, Naser Amini


A number of lifestyle-related factors, including smoking and obesity, as well as a number of environmental factors, such as exposure to dioxins, combustion products, and traffic exhaust gases, appear to have a deleterious influence on the testes and spermatogenesis.

Because of its Oxidation-Reduction (Redox) Potential, copper functions as a cofactor in a variety of enzymes that are essential for basic metabolic functions. We assess how CuO-NPs affected the male reproductive system and the probability of getting pregnant, both of which have not yet been studied. Four groups of the creatures were chosen at random, totaling six. The control group (group 1) received no assistance. The other groups (groups 2, 3, and 4) received daily doses of 50, 100, and 150 mg/kg of CuO-NP dissolved in 1 ml of distilled water. For one day, all injections were intraperitoneal (IP). The epididymis had thicker tunica and tubes, according to the results of the histological investigation (p< 0.05). CuO2 NP treatment significantly enhanced Sertoli and Leydig cells as well as all testicular germ cells other than spermatozoa at a dose of 100 mg/kg in both the in-vivo and in-vitro stages. The 150 mg group's spermatids and spermatocytes had significantly decreased as a result of CuO2 NP's harmful effects during the in-vitro phase (P 0.05). We can therefore conclude that a buildup of Cu in high doses may result in a disruption of the Cu homeostasis, which may subsequently reduce male fertility.


key words: CUO2, Nanoparticle, Spermatogenesis


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