Email this article Evaluation of serum alkaline phosphatase and calcium in type 2 diabetes mellitus patients at Lucknow, India
Abstract
There is an increasing demand and requirement to develop new biomarkers for the early diagnosis and detection of diabetes mellitus (DM). The present study is designed to estimate serum alkaline phosphatase (ALP) and serum calcium levels in type 2 diabetes mellitus (T2DM) patients. The serum alkaline phosphatase and calcium levels were estimated using commercially available kits on a semiautoanalyzer. The fasting blood sugar (FBS) of the subjects was measured by the glucose oxidase-peroxidase (GOD-POD) method using a commercially available kit. The level of ALP was found to be statistically significant in T2DM patients when compared with matched healthy individuals (p <0.001), whereas the association of serum calcium level was found to be non-significant (p = 0.07). Although there is a decrease in serum calcium level in T2DM patients when compared with healthy controls. It is concluded that the management of T2DM and its associated complications may require regular estimation of serum ALP and calcium levels. The estimations may also be significant in the management of osteoporosis in T2DM patients. High utilization of calcium and vitamin D especially from supplements may lower the risk of DM.
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Kanwar G, Jain N, Sharma N, Shekhawat M, Ahmed J, Kabra R. Significance of serum urea and creatinine levels in type 2 diabetic patients. IOSR J Dent Med Sci. 2015; 14(8):65-7.
Standards of medical care in diabetes--2011. Diabetes Care. 2011; 34 Suppl 1(Suppl 1):S11-61.
Kumar A, Goel MK, Jain RB, Khanna P, Chaudhary V. India towards diabetes control: Key issues. Australas Med J. 2013; 6(10):524-31.
Rigo J, Mohamed M, De Curtis M. Disorders of calcium, phosphorus and magnesium metabolism. Neonatal-Perinatal medicine: disease of the fetus and infanth. 2006; 8:1492-523.
Yu, E.; Sharma, S. Physiology, Calcium. In StatPearls; StatPearls Publishing: Treasure Island, FL, USA, 2022.
Wei W, Jiang W, Gu W, Wu H, Jiang H, Li G, et al. The joint effect of energy reduction with calcium supplementation on the risk factors of type 2 diabetes in the overweight population: a two-year randomized controlled trial. Aging (Albany NY). 2021; 13(4):5571-84.
Yamada H, Funazaki S, Suzuki D, Saikawa R, Yoshida M, Kakei M, et al. Association between Urinary Calcium Excretion and Estimated Glomerular Filtration Rate Decline in Patients with Type 2 Diabetes Mellitus: A Retrospective Single-center Observational Study. J Clin Med. 2018; 7(7):171.
Seino Y, Nanjo K, Tajima N, Kadowaki T, Kashiwagi A, Araki E, et al. Report of the committee on the classification and diagnostic criteria of diabetes mellitus. J Diabetes Investig. 2010; 1(5):212-28.
Rani DP, Anandan SN. A clinical study of serum alkaline phosphatase and calcium level in type 2 diabetes mellitus with periodontitis among the south Indian population. SRM J Res Dent Sci. 2012; 3(3):175.
Trinder P. Enzymatic determination of glucose. Ann Clin Biochem. 1969; 6:24-7.
Schiele F, Muller J, Colinet E, Siest G. Certification of an enzyme reference material for alkaline phosphatase (CRM 371). Clin Biochem. 1991; 24(2):159-68.
McComb RB, Bowers GN, Jr. Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum. Clin Chem. 1972; 18(2):97-104.
Recommendations of the German Society for Clinical Chemistry. Standardisation of methods for the estimation of enzyme activities in biological fluids. Experimental basis for the optimized standard conditions. Z Klin Chem Klin Biochem. 1972; 10(6):281-91.
Gitelman HJ. An improved automated procedure for the determination of calcium in biological specimens. Anal Biochem. 1967; 18(3):521-31.
Baginski ES, Marie SS, Clark WL, Zak B. Direct microdetermination of serum calcium. Clin Chim Acta. 1973; 46(1):49-54.
Marshall D, Johnell O, Wedel H. Meta-analysis of how well measures of bone mineral density predict occurrence of osteoporotic fractures. BMJ. 1996; 312(7041):1254-9.
Nordin, BEC. Calcium, Phosphate and Magnesium Metabolism: Clinical Physiology and Diagnostic Procedures. Edinburgh: Churchill Livingstone, 1976.
Edwards SL. Maintaining calcium balance: physiology and implications. Nurs Times. 2005; 101(19):58-61.
Robinson LJ, Blair HC, Barnett JB, Zaidi M, Huang CL. Regulation of bone turnover by calcium-regulated calcium channels. Ann N Y Acad Sci. 2010; 1192:351-7.
Nagasaka S, Murakami T, Uchikawa T, Ishikawa SE, Saito T. Effect of glycemic control on calcium and phosphorus handling and parathyroid hormone level in patients with non-insulin-dependent diabetes mellitus. Endocr J. 1995; 42(3):377-83.
Tsavaris NB, Pangalis GA, Variami E, Karabelis A, Kosmidis P, Raptis S. Association of neutrophil alkaline phosphatase activity and glycosylated haemoglobin in diabetes mellitus. Acta Haematol. 1990; 83(1):22-5.
Sultan E, Taha I, Saber LM. Altered bone metabolic markers in type 2 diabetes mellitus: Impact of glycemic control. J Taibah Univ Medical Sci. 2008; 3(2):104-16.
Hofbauer LC, Brueck CC, Singh SK, Dobnig H. Osteoporosis in patients with diabetes mellitus. J Bone Miner Res. 2007; 22(9):1317-28.
Botolin S, McCabe LR. Chronic hyperglycemia modulates osteoblast gene expression through osmotic and non-osmotic pathways. J Cell Biochem. 2006; 99(2):411-25. Sun G, Vasdev S, Martin GR, Gadag V, Zhang H. Altered calcium homeostasis is correlated with abnormalities of fasting serum glucose, insulin resistance, and beta-cell function in the Newfoundland population. Diabetes. 2005; 54(11):3336-9.
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