<ArticleSet>
<Article>
<Journal>
<PublisherName></PublisherName>
<JournalTitle>J Curr Biomed Rep</JournalTitle>
<Volume>1</Volume>
<Issue>1</Issue>
<PubDate>
<Year>2020</Year>
<Month>08</Month>
<Day>05</Day>
</PubDate>
</Journal>
<ArticleTitle>Molecular investigation of extended-spectrum β-lactamases (ESBLs) genes in the Salmonella isolates obtained from children with acute diarrhea</ArticleTitle>
<FirstPage>17</FirstPage>
<LastPage>22</LastPage>
<Language>EN</Language>
<AuthorList>
<Author>
<FirstName>Mohsen</FirstName>
<LastName>Mohammadi</LastName>
<Affiliation>Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. abazar_pournajaf@yahoo.com</Affiliation>
</Author>
<Author>
<FirstName>Mohammad Hossein</FirstName>
<LastName>Tayefeh-Arbab</LastName>
</Author>
<Author>
<FirstName>Zohre</FirstName>
<LastName>Baseri</LastName>
</Author>
<Author>
<FirstName>Mojtaba</FirstName>
<LastName>Taghizadeh Armaki</LastName>
</Author>
<Author>
<FirstName>Mohsen</FirstName>
<LastName>Karami</LastName>
</Author>
<Author>
<FirstName>Saman</FirstName>
<LastName>Alhooei</LastName>
</Author>
<Author>
<FirstName>Abazar</FirstName>
<LastName>Pournajaf</LastName>
</Author>
</AuthorList>
<History>
<PubDate>
<Year>2020</Year>
<Month>07</Month>
<Day>05</Day>
</PubDate>
<PubDate>
<Year>2020</Year>
<Month>08</Month>
<Day>01</Day>
</PubDate>
<PubDate>
<Year>2020</Year>
<Month>08</Month>
<Day>01</Day>
</PubDate>
</History>
<Abstract>Salmonellosis is an important public health concern among children in worldwide. Extended-spectrum β-lactams (ESBLs) cause resistance to clinically important beta-lactams which are generally used to treat invasive Salmonella infections. Therefore, the aim of this study was to investigate the presence of SHV, TEM and CTX-M genes in different strains of Salmonella isolated from children with acute diarrhea and to determine their resistance profile. In this cross-sectional study, 300 fecal samples were collected from children referred to the Amirkola Children's Hospital, Babol, Iran. Antibiotic susceptibility testing was done according to the CLSI guideline. ESBLs-producing strains were identified using double disk synergy test method on the Mueller-Hinton agar plates. Multiplex-PCR was performed using oligonucleotide specific primers to detect of SHV, TEM and CTX-M genes. In total, 7% (n; 21/300) salmonella were isolated, which 61.9%, 28.6% and 9.5% were Salmonella typhimurium, Salmonella enteritidis and Salmonella typhi, respectively. The prevalence of the ESBL-producing isolates were 52.4%. M-PCR results showed that 42.8%, 38.1% and 14.3% of isolates were carried CTX-M, TEM and SHV genes, respectively. Also, 18.2% of isolates harbored CTX-M, and TEM genes, simultaneously. The high rate of ESBLs-producing Salmonella strains in the pediatric patients is an alarm. It is also recommended that alternative drugs be used with less resistance, which requires further investigation.</Abstract>
<ObjectList>
<Object>
<Param>Beta-lactamase</Param>
</Object>
<Object>
<Param>Salmonella</Param>
</Object>
<Object>
<Param>Diarrhea</Param>
</Object>
<Object>
<Param>Pediatric</Param>
</Object>
<Object>
<Param>PCR</Param>
</Object>
</ObjectList>
</Article>
</ArticleSet>

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