Use of nanotechnology in the diagnosis and treatment of coronavirus

Elham Maghareh Abed, Seyedeh Mahsan Hoseini-Alfatemi, Hoda Sabati, Mohammad Amin Khajavi Gaskarei, Kourosh Delpasand, Marzie Ghasemi


Coronavirus is a beta virus that has caused a worldwide pandemic since December 2019. Many treatments such as antiviral drugs, immunosuppressive drugs, neutralizing antibodies, and monoclonal antibodies have been tested on coronavirus disease 2019 (COVID-19) that most of them were effective. Given that nanotechnology-based approaches have been successful in detection and treatment of viral systems such as human immunodeficiency virus (HIV), influenza A virus subtype H1N1 and Middle East respiratory syndrome coronavirus (MERS-CoV), they also seem to be effective in detecting and treating COVID-19. Nanotechnology is used in various methods for early and rapid diagnosis of the disease. Nanoparticles can be used in products for the diagnosis, treatment and prevention of COVID-19. These substances are very effective in the controlled delivery of antiviral drugs and biomolecules and they are also used in the manufacture of personal safety equipment, widely, and the production of anti-virus coatings for surfaces, air filters and the production of vaccines. In general, nanomaterial can play an important role in controlling the disease, based on strategies to prevent the virus from entering the host cell, inhibiting virus replication, virus delivery systems, and nano-based vaccines. Nanotechnology is a multidisciplinary tool that can offer a variety of solutions based on disease prevention, diagnosis and treatment strategies.


COVID-19; Nanotechnology; Coronavirus; Diagnosis; Treatment


Rehman SU, Shafique L, Ihsan A, Liu Q. Evolutionary Trajectory for the Emergence of Novel Coronavirus SARS-CoV-2. Pathogens. 2020; 9(3):240.

Ruiz-Hitzky E, Darder M, Wicklein B, Ruiz-Garcia C, Martín-Sampedro R, Del Real G, et al. Nanotechnology Responses to COVID-19. Adv Healthc Mater. 2020; 9(19):e2000979.

Ou X, Liu Y, Lei X, Li P, Mi D, Ren L, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun. 2020; 11(1):1620.

Zumla A, Chan JF, Azhar EI, Hui DS, Yuen KY. Coronaviruses - drug discovery and therapeutic options. Nat Rev Drug Discov. 2016; 15(5):327-47.

Khan M, Adil SF, Alkhathlan HZ, Tahir MN, Saif S, Khan M, et al. COVID-19: A Global Challenge with Old History, Epidemiology and Progress So Far. Molecules. 2020; 26(1):39.

Forster P, Forster L, Renfrew C, Forster M. Phylogenetic network analysis of SARS-CoV-2 genomes. Proc Natl Acad Sci USA. 2020; 117(17):9241-3.

Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, et al. Clinical Characteristics of Coronavirus Disease 2019 in China. N Engl J Med. 2020; 382(18):1708-20.

Tang Z, Zhang X, Shu Y, Guo M, Zhang H, Tao W. Insights from nanotechnology in COVID-19 treatment. Nano Today. 2021; 36:101019.

Walls AC, Park YJ, Tortorici MA, Wall A, McGuire AT, Veesler D. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell. 2020; 181(2):281-92.e6.

Tian X, Li C, Huang A, Xia S, Lu S, Shi Z, et al. Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody. Emerg Microbes Infect. 2020; 9(1):382-5.

Mizumoto K, Kagaya K, Zarebski A, Chowell G. Estimating the asymptomatic proportion of coronavirus disease 2019 (COVID-19) cases on board the Diamond Princess cruise ship, Yokohama, Japan, 2020. Euro Surveill. 2020; 25(10):2000180.

Grein J, Ohmagari N, Shin D, Diaz G, Asperges E, Castagna A, et al. Compassionate Use of Remdesivir for Patients with Severe Covid-19. N Engl J Med. 2020; 382(24):2327-36.

Bhavana V, Thakor P, Singh SB, Mehra NK. COVID-19: Pathophysiology, treatment options, nanotechnology approaches, and research agenda to combating the SARS-CoV2 pandemic. Life Sci. 2020; 261:118336.

UK researchers launch trial of blood plasma therapy for Covid-19. UK researchers launch trial of blood plasma therapy for Covid-19 2020 [updated May 4th, 2020]. Available at:

Jindal S, Gopinath P. Nanotechnology based approaches for combatting COVID-19 viral infection. Nano Express. 2020 1(2).

Chauhan G, Madou MJ, Kalra S, Chopra V, Ghosh D, Martinez-Chapa SO. Nanotechnology for COVID-19: Therapeutics and Vaccine Research. ACS Nano. 2020; 14(7):7760-82.

Luo F, Long C, Wu Z, Xiong H, Chen M, Zhang X, et al. Functional silica nanospheres for sensitive detection of H9N2 avian influenza virus based on immunomagnetic separation. Sens Actuators B Chem. 2020; 310:127831.

Singh R, Hong S, Jang J. Label-free detection of influenza viruses using a reduced graphene oxide-based electrochemical immunosensor integrated with a microfluidic platform. Sci Rep. 2017; 7:42771.

Lara HH, Ayala-Nuñez NV, Ixtepan-Turrent L, Rodriguez-Padilla C. Mode of antiviral action of silver nanoparticles against HIV-1. J Nanobiotechnology. 2010; 8:1.

Yang X, Li C, Huang C. Curcumin modified silver nanoparticles for highly efficient inhibition of respiratory syncytial virus infection. Nanoscale. 2016; 8(5):3040-8.

Mhlwatika Z, Aderibigbe B. Application of dendrimers for the treatment of infectious diseases. Molecules. 2018; 23(9):2205.

Pollock S, Nichita N, Böhmer A, Radulescu C, Dwek R, Zitzmann N. Polyunsaturated liposomes are antiviral against hepatitis B and C viruses and HIV by decreasing cholesterol levels in infected cells. Proc Natl Acad Sci U S A. 2010; 107(40):17176-81.

Wang N, Wei C, Zhang Z, Liu T, Wang T. Aluminum Nanoparticles Acting as a Pulmonary Vaccine Adjuvant-Delivery System (VADS) Able to Safely Elicit Robust Systemic and Mucosal Immunity. J Inorg Organomet Polym Mater. 2020; 30:4203-17.

Cardoso VMdO, Moreira BJ, Comparetti EJ, Sampaio I, Ferreira LMB, Lins PMP, et al. Is Nanotechnology Helping in the Fight Against COVID-19? Frontiers in Nanotechnology. 2020; 2(4):588915.

Weiss C, Carriere M, Fusco L, Capua I, Regla-Nava JA, Pasquali M, et al. Toward Nanotechnology-Enabled Approaches against the COVID-19 Pandemic. ACS Nano. 2020; 14(6):6383-406.

Nurul Najian AB, Engku Nur Syafirah EA, Ismail N, Mohamed M, Yean CY. Development of multiplex loop mediated isothermal amplification (m-LAMP) label-based gold nanoparticles lateral flow dipstick biosensor for detection of pathogenic Leptospira. Anal Chim Acta. 2016; 903:142-8.

Mozhgani SH, Kermani HA, Norouzi M, Arabi M, Soltani S. Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection. Heliyon. 2020; 6(5):e04048.

Kim H, Park M, Hwang J, Kim JH, Chung DR, Lee KS, et al. Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles. ACS Sens. 2019; 4(5):1306-12.

Zhu X, Wang X, Han L, Chen T, Wang L, Li H, et al. Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19. Biosens Bioelectron. 2020; 166:112437.

Huang Y, Xu T, Wang W, Wen Y, Li K, Qian L, et al. Lateral flow biosensors based on the use of micro- and nanomaterials: a review on recent developments. Mikrochim Acta. 2019; 187(1):70.

Layqah LA, Eissa S. An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes. Mikrochim Acta. 2019; 186(4):224.

Amanat F, Krammer F. SARS-CoV-2 Vaccines: Status Report. Immunity. 2020; 52(4):583-9.

Chen WH, Strych U, Hotez PJ, Bottazzi ME. The SARS-CoV-2 Vaccine Pipeline: an Overview. Curr Trop Med Rep. 2020:1-4.

Kerry RG, Malik S, Redda YT, Sahoo S, Patra JK, Majhi S. Nano-based approach to combat emerging viral (NIPAH virus) infection. Nanomedicine. 2019; 18:196-220.

Khanal M, Vausselin T, Barras A, Bande O, Turcheniuk K, Benazza M, et al. Phenylboronic-acid-modified nanoparticles: potential antiviral therapeutics. ACS Appl Mater Interfaces. 2013; 5(23):12488-98.

Łoczechin A, Séron K, Barras A, Giovanelli E, Belouzard S, Chen YT, et al. Functional Carbon Quantum Dots as Medical Countermeasures to Human Coronavirus. ACS Appl Mater Interfaces. 2019; 11(46):42964-74.

Huang X, Li M, Xu Y, Zhang J, Meng X, An X, et al. Novel Gold Nanorod-Based HR1 Peptide Inhibitor for Middle East Respiratory Syndrome Coronavirus. ACS Appl Mater Interfaces. 2019; 11(22):19799-807.

Du T, Liang J, Dong N, Lu J, Fu Y, Fang L, et al. Glutathione-Capped Ag(2)S Nanoclusters Inhibit Coronavirus Proliferation through Blockage of Viral RNA Synthesis and Budding. ACS Appl Mater Interfaces. 2018; 10(5):4369-78.

Ghaffari H, Tavakoli A, Moradi A, Tabarraei A, Bokharaei-Salim F, Zahmatkeshan M, et al. Inhibition of H1N1 influenza virus infection by zinc oxide nanoparticles: another emerging application of nanomedicine. J Biomed Sci. 2019; 26(1):70.

Razzaque MS. COVID-19 Pandemic: Can Maintaining Optimal Zinc Balance Enhance Host Resistance? Tohoku J Exp Med. 2020; 251(3):175-81.

Kobayashi K, Wei J, Iida R, Ijiro K, Niikura K. Surface engineering of nanoparticles for therapeutic applications. Polymer J. 2014; 46(8):460-8.

Milovanovic M, Arsenijevic A, Milovanovic J, Kanjevac T, Arsenijevic N. Chapter 14 - Nanoparticles in Antiviral Therapy. In: Grumezescu AM, editor. Antimicrobial Nanoarchitectonics: Elsevier; 2017. p. 383-410.

Chang D, Xu H, Rebaza A, Sharma L, Dela Cruz CS. Protecting health-care workers from subclinical coronavirus infection. Lancet Respir Med. 2020; 8(3):e13.

Warnes SL, Little ZR, Keevil CW. Human Coronavirus 229E Remains Infectious on Common Touch Surface Materials. mBio. 2015; 6(6):e01697-15.

Kampf G, Todt D, Pfaender S, Steinmann E. Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents. J Hosp Infect. 2020; 104(3):246-51.

Ong S, Tan Y, Chia P, Lee T, Ng O, Wong M, et al. Air, surface environmental, and personal protective equipment contamination by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from a symptomatic patient. JAMA. 2020; 323(16):1610-2.

Murphy F, Tchetchik A, Furxhi I. Reduction of Health Care-Associated Infections (HAIs) with Antimicrobial Inorganic Nanoparticles Incorporated in Medical Textiles: An Economic Assessment. Nanomaterials (Basel). 2020; 10(5):999.

Palza H, Nuñez M, Bastías R, Delgado K. In situ antimicrobial behavior of materials with copper-based additives in a hospital environment. Int J Antimicrob Agents. 2018; 51(6):912-7.

Iyigundogdu ZU, Demir O, Asutay AB, Sahin F. Developing Novel Antimicrobial and Antiviral Textile Products. Appl Biochem Biotechnol. 2017; 181(3):1155-66.

Akhavan O, Choobtashani M, Ghaderi E. Protein degradation and RNA efflux of viruses photocatalyzed by graphene–tungsten oxide composite under visible light irradiation. J Phys Chem C. 2012; 116(17):9653-9.

Balagna C, Perero S, Percivalle E, Nepita EV, Ferraris M. Virucidal effect against coronavirus SARS-CoV-2 of a silver nanocluster/silica composite sputtered coating. Open Ceram. 2020; 1:100006.

Nakashima R, Kawamoto M, Miyazaki S, Onishi R, Furusaki K, Osaki M, et al. Evaluation of calcium hydrogen carbonate mesoscopic crystals as a disinfectant for influenza A viruses. J Vet Med Sci. 2017; 79(5):939-42.

Sakudo A, Yamashiro R, Haritani M, Furusaki K, Onishi R, Onodera T. Inactivation of Non-Enveloped Viruses and Bacteria by an Electrically Charged Disinfectant Containing Meso-Structure Nanoparticles via Modification of the Genome. Int J Nanomedicine. 2020; 15:1387-95.

Pfaff F, Glück B, Hoyer T, Rohländer D, Sauerbrei A, Zell R. Tungsten carbide nanoparticles show a broad spectrum virucidal activity against enveloped and nonenveloped model viruses using a guideline‐standardized in vitro test. Lett Appl Microbiol. 2019; 69:302-9.

Deshmukh SP, Patil SM, Mullani SB, Delekar SD. Silver nanoparticles as an effective disinfectant: A review. Mater Sci Eng C Mater Biol Appl. 2019; 97:954-65.

Galdiero S, Falanga A, Vitiello M, Cantisani M, Marra V, Galdiero M. Silver nanoparticles as potential antiviral agents. Molecules. 2011; 16(10):8894-918.

Noor N, Mutalik S, Younas MW, Chan CY, Thakur S, Wang F, et al. Durable Antimicrobial Behaviour from Silver-Graphene Coated Medical Textile Composites. Polymers (Basel). 2019; 11(12):2000.

Kostarelos K. Nanoscale nights of COVID-19. Nat Nanotechnol. 2020; 15(5):343-4.

Lurie N, Saville M, Hatchett R, Halton J. Developing Covid-19 Vaccines at Pandemic Speed. N Engl J Med. 2020; 382(21):1969-73.

Park YM, Lee SJ, Kim YS, Lee MH, Cha GS, Jung ID, et al. Nanoparticle-based vaccine delivery for cancer immunotherapy. Immune Netw. 2013; 13(5):177-83.

Graham RL, Becker MM, Eckerle LD, Bolles M, Denison MR, Baric RS. A live, impaired-fidelity coronavirus vaccine protects in an aged, immunocompromised mouse model of lethal disease. Nat Med. 2012; 18(12):1820-6.

Steinman RM, Banchereau J. Taking dendritic cells into medicine. Nature. 2007; 449(7161):419-26.

Lohcharoenkal W, Wang L, Chen YC, Rojanasakul Y. Protein nanoparticles as drug delivery carriers for cancer therapy. Biomed Res Int. 2014; 2014:180549.

Dykman LA. Gold nanoparticles for preparation of antibodies and vaccines against infectious diseases. Expert Rev Vaccines. 2020; 19(5):465-77.


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