Email this article Molecular investigation of extended-spectrum β-lactamases (ESBLs) genes in the Salmonella isolates obtained from children with acute diarrhea
Abstract
Salmonellosis is an important public health concern among children in worldwide. Extended-spectrum β-lactams (ESBLs) cause resistance to clinically important beta-lactams which are generally used to treat invasive Salmonella infections. Therefore, the aim of this study was to investigate the presence of SHV, TEM and CTX-M genes in different strains of Salmonella isolated from children with acute diarrhea and to determine their resistance profile. In this cross-sectional study, 300 fecal samples were collected from children referred to the Amirkola Children's Hospital, Babol, Iran. Antibiotic susceptibility testing was done according to the CLSI guideline. ESBLs-producing strains were identified using double disk synergy test method on the Mueller-Hinton agar plates. Multiplex-PCR was performed using oligonucleotide specific primers to detect of SHV, TEM and CTX-M genes. In total, 7% (n; 21/300) salmonella were isolated, which 61.9%, 28.6% and 9.5% were Salmonella typhimurium, Salmonella enteritidis and Salmonella typhi, respectively. The prevalence of the ESBL-producing isolates were 52.4%. M-PCR results showed that 42.8%, 38.1% and 14.3% of isolates were carried CTX-M, TEM and SHV genes, respectively. Also, 18.2% of isolates harbored CTX-M, and TEM genes, simultaneously. The high rate of ESBLs-producing Salmonella strains in the pediatric patients is an alarm. It is also recommended that alternative drugs be used with less resistance, which requires further investigation.
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References
Gordon MA, Feasey NA, Graham SM. 45 - Nontyphoid Salmonella Disease. In: Magill AJ, Hill DR, Solomon T, Ryan ET, editors. Hunter's Tropical Medicine and Emerging Infectious Disease (Ninth Edition). London: W.B. Saunders; 2013. p. 462-7.
Gast, RK and Porter, RE., Jr. Salmonella Infections. In Diseases of Poultry. (eds D.E. Swayne, M. Boulianne, CM. Logue, LR. McDougald, V. Nair, DL. Suarez, S. Wit, T. Grimes, D. Johnson, M. Kromm, TY. Prajitno, I. Rubinoff and G. Zavala). 2020. p. 717-53.
Sevilla-Navarro S, Catalá-Gregori P, García C, Cortés V, Marin C. Salmonella Infantis and Salmonella Enteritidis specific bacteriophages isolated form poultry faeces as a complementary tool for cleaning and disinfection against Salmonella. Comp Immunol Microb. 2020; 68:101405.
Kadhim HM. Review of pathogenicity and virulence determinants in Salmonella. EurAsian J Biosci. 2020; 14(1):377-81.
Lee CM, Lee MS, Yang TL, Lee KL, Yen TY, Lu CY, et al. Clinical features and risk factors associated with bacteremia of nontyphoidal salmonellosis in pediatric patients, 2010-2018. J Formos Med Assoc. 2020. [In press]. doi: 10.1016/j.jfma.2020.04.022.
Lingzhi L, Haojie G, Dan G, Hongmei M, Yang L, Mengdie J, et al. The role of two-component regulatory system in β-lactam antibiotics resistance. Microbiol Res. 2018; 215:126-9.
AL_Jubori SS, AL_Kadmy IM, JassimAl_Ani Z. Emergence of multidrug resistance (MDR) Acinetobacter baumannii isolated from Iraqi hospitals. Adv Environ Biol. 2016; 10(5):265-76.
Bush K, Jacoby GA. Updated functional classification of beta-lactamases. Antimicrob Agents Chemother. 2010; 54(3):969-76.
Bush K, Bradford PA. Epidemiology of β-Lactamase-Producing Pathogens. Clin Microbiol Rev. 2020; 33(2):e00047-19.
Sawa T, Kooguchi K, Moriyama K. Molecular diversity of extended-spectrum β-lactamases and carbapenemases, and antimicrobial resistance. J Intensive Care. 2020; 8:13.
Kwok KO, Chan E, Chung PH, Tang A, Wei WI, Zhu C, et al. Prevalence and associated factors for carriage of Enterobacteriaceae producing ESBLs or carbapenemase and methicillin-resistant Staphylococcus aureus in Hong Kong community. J Infect. 2020; 81(2):242-7.
Kawamura K, Sugawara T, Matsuo N, Hayashi K, Norizuki C, Tamai K, et al. Spread of CTX-Type Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates of Epidemic Clone B2-O25-ST131 Among Dogs and Cats in Japan. Microb Drug Resist. 2017; 23(8):1059-66.
CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute, 2019.
Gajdács M, Urbán E. Epidemiological Trends and Resistance Associated with Stenotrophomonas maltophilia Bacteremia: A 10-Year Retrospective Cohort Study in a Tertiary-Care Hospital in Hungary. Diseases. 2019; 7(2):41.
Nobari S, Shahcheraghi F, Rahmati Ghezelgeh F, Valizadeh B. Molecular characterization of carbapenem-resistant strains of Klebsiella pneumoniae isolated from Iranian patients: first identification of blaKPC gene in Iran. Microb Drug Resist. 2014; 20(4):285-93.
Monstein HJ, Ostholm-Balkhed A, Nilsson MV, Nilsson M, Dornbusch K, Nilsson LE. Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. Apmis. 2007; 115(12):1400-8.
Ghasemi Y, Archin T, Kargar M, Mohkam M. A simple multiplex PCR for assessing prevalence of extended-spectrum β-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz, Iran. Asian Pac J Trop Med. 2013; 6(9):703-8.
Nodeh Farahani N, Masjedian Jazi F, Nikmanesh B, Asadolahi P, Sadeghi Kalani B, Amirmozafari N. Prevalence and Antibiotic Susceptibility Patterns of Salmonella and Shigella Species Isolated from Pediatric Diarrhea in Tehran. Arch Pediatr Infect Dis. 2018; 6(4):e57328.
Soltan Dallal MM, Rastegar Lari A, Sharifi Yazdi MK. [Pattern of serotyping and antibiotic resistance of Salmonella in children with diarrhea]. J Gorgan Uni Med Sci. 2014; 16(1): 100-105. [In Persian]
Eshraghi S, Dalall MMS, Fardsanei F, Salehi TZ, Ranjbar R, Nikmanesh B, et al. Salmonella enteritidis and antibiotic resistance patterns: A study on 1950 children with diarrhea. Tehran Univ Med J. 2010; 67(12):876-82.
Siourimè SN, Isidore BOJ, Oumar T, Nestor BIH, Yves T, Nicolas B, et al. Serotyping and antimicrobial drug resistance of Salmonella isolated from lettuce and human diarrhea samples in Burkina Faso. Afr J Infect Dis. 2017; 11(2):24-30.
Spiliopoulou I, Zografou S, Goula A, Dimitracopoulos G, Christofidou M. Molecular epidemiology and antibiotic resistance patterns of Salmonella enterica from southwestern Greece. Chemotherapy. 2007; 53(6):392-6.
Tajbakhsh M, Avini MY, Khajeh JA, Alebouyeh M, Mojarad EN, Zali MR. Increased-resistance phenotype resulted from elevated β-lactamase enzyme activity in Salmonella clinical isolates. J Isfahan Med Sch. 2012; 30(178).
Ranjbar R, Ardashiri M, Samadi S, Afshar D. Distribution of extended-spectrum β-lactamases (ESBLs) among Salmonella serogroups isolated from pediatric patients. Iran J Microbiol. 2018; 10(5):294-9.
Boisramé-Gastrin S, Tandé D, Münck MR, Gouriou S, Nordmann P, Naas T. Salmonella carriage in adopted children from Mali: 2001-08. J Antimicrob Chemother. 2011; 66(10):2271-6.
DOI: https://doi.org/10.52547/jcbior.1.1.17
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